YMC BioPro IEX
YMC BioPro IEX Media and Preparative Columns from YMC Europe GMBH
Ion exchange chromatography (IEX) is widely used in the analysis and purification of bio-molecules. Using reversible charge-charge interactions offers several advantages in comparison to other chromatographic methods, e.g. high capacity and fast throughput. For these reasons, IEX is often used in the capture or intermediate purification of bio-molecules.
BioPro is a series of ion exchange resins specifically designed for use in bio-chromatography. The media is based on a hydrophilic polymer matrix, with particle sizes of 10, 20, 30, or 75 µm. It is available as a strong anion exchanger (BioPro QA) or a strong cation exchanger (BioPro SP). BioPro offers a high dynamic binding capacity (DBC), together with low non-specific adsorption and excellent recovery.
The BioPro Ion Exchange Screening Kits consist of columns that are packed with resins designed for the separation of proteins, nucleotides, and other biomolecules. The available phases are strong anion exchanger (BioPro SmartSep Q), strong cation exchanger (BioPro SmartSep S), weak anion exchanger (BioPro DA) and weak cation exchanger (BioPro CM).
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- Features
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- High dynamic binding capacity
- Low non-specific adsorption
- Excellent recovery
- Easy removal
- Cleaning-in-place with NaOH solutions applicable
- Specifications
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BioPro SmartSep for intermediate purifications and polishing
BioPro SmartSepQ10BioPro SmartSepQ20BioPro SmartSepQ30BioPro SmartSepS10BioPro SmartSepS20BioPro SmartSepS30Ion exchange type strong anion exchanger strong cation exchanger Charged group -CH2N+(CH3)3 -(CH2)4SO3- Matrix hydrophilic polymer beads Pore Size porous Compression factor 1.1 - 1.4 Particle size 10 µm 20 µm 30 µm 10 µm 20 µm 30 µm Pressure resistance Regular use: 30 bar Max: 40 barRegular use: 20 bar Max: 30 barRegular use: 30 bar Max: 40 barRegular use: 20 bar Max: 30 barIon-exchange capacity min. 0.08 meq/mL resin min. 0.08 meq/mL resin Dynamic binding capacity min. 100 mg/mL resin (BSA) min. 100 mg/mL resin (lysozyme) BioPro for capture
BioPro Q75 BioPro S75 Ion exchange type strong anion exchanger strong cation exchanger Charged group -R-N+(CH3)3 -R-SO3- Matrix hydrophilic polymer beads Pore Size porous Compression factor 1.1 - 1.4 Particle size 75 µm Pressure resistance 3 bar Ion-exchange capacity min. 0.10 meq/mL resin Dynamic binding capacity min. 160 mg/mL resin (BSA) BioPro preparative screening kits
BioPro SmartSep QBioPro SmartSep SBioPro DABioPro CMIon exchange type strong anion exchanger strong cation exchanger weak anion exchanger weak cation exchanger Charged group -CH2N+(CH3)3 -CH2CH2CH2CH2SO3- -R-N(CH3)2 -R-COOH Matrix porous hydrophilic polymer porous methacrylate polymer Particle size 30, 75 µm 60 µm pH range 2 - 12 Regular use: 3 - 12
Short term: 1 - 13 - Literature
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YMC General Catalogue
Contains information on the full range of YMC columns
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YMC Analytical Column Overview
Specifications of the full range of YMC columns
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